The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.
Identifieur interne : 000444 ( Main/Exploration ); précédent : 000443; suivant : 000445The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.
Auteurs : Liying Sun [République populaire de Chine] ; Ida Bagus Andika ; Jiangfeng Shen ; Di Yang ; Claudio Ratti ; Jianping ChenSource :
- Virus research [ 1872-7492 ] ; 2013.
Descripteurs français
- KwdFr :
- Alignement de séquences (MeSH), Données de séquences moléculaires (MeSH), Initiation de la traduction (MeSH), Liaison aux protéines (MeSH), Maladies des plantes (virologie), Protéines de capside (génétique), Protéines de capside (métabolisme), Suppression génétique (MeSH), Séquence d'acides aminés (MeSH), Séquence nucléotidique (MeSH), Triticum (virologie), Virus à ARN (génétique), Virus à ARN (isolement et purification), Virus à ARN (métabolisme).
- MESH :
- génétique : Protéines de capside, Virus à ARN.
- isolement et purification : Virus à ARN.
- métabolisme : Protéines de capside, Virus à ARN.
- virologie : Maladies des plantes, Triticum.
- Alignement de séquences, Données de séquences moléculaires, Initiation de la traduction, Liaison aux protéines, Suppression génétique, Séquence d'acides aminés, Séquence nucléotidique.
English descriptors
- KwdEn :
- Amino Acid Sequence (MeSH), Base Sequence (MeSH), Capsid Proteins (genetics), Capsid Proteins (metabolism), Molecular Sequence Data (MeSH), Peptide Chain Initiation, Translational (MeSH), Plant Diseases (virology), Protein Binding (MeSH), RNA Viruses (genetics), RNA Viruses (isolation & purification), RNA Viruses (metabolism), Sequence Alignment (MeSH), Suppression, Genetic (MeSH), Triticum (virology).
- MESH :
- chemical , genetics : Capsid Proteins.
- chemical , metabolism : Capsid Proteins.
- genetics : RNA Viruses.
- isolation & purification : RNA Viruses.
- metabolism : RNA Viruses.
- virology : Plant Diseases, Triticum.
- Amino Acid Sequence, Base Sequence, Molecular Sequence Data, Peptide Chain Initiation, Translational, Protein Binding, Sequence Alignment, Suppression, Genetic.
Abstract
Some viruses use alternative translation initiation at non-AUG codons as a strategy to produce multiple proteins during gene expression. Here we show that, using this strategy, Chinese wheat mosaic virus (CWMV; Furovirus) expresses a larger form of coat protein (N-ext/CP) in infected plants. Site-directed mutagenesis and transient expression analysis confirmed that CWMV N-ext/CP is initiated at an upstream in-frame CUG codon at nucleotide position 207-209 of RNA 2, which adds a 39 amino acid (aa) N-terminal extension to the major CP. Interestingly, in planta and in vitro analyses indicated that CWMV N-ext/CP but not CP interacts with the CWMV cysteine-rich protein (CRP), an RNA silencing suppressor. We further determined that the N-terminal 39 aa extension, particularly the 10 aa region immediately upstream of the major CP coding region is responsible for the interaction of N-ext/CP with CRP. In an Agrobacterium co-infiltration assay, co-expression with N-ext/CP did not affect CRP silencing suppression activity. Thus the alternative translation initiation at a CUG codon provides the CWMV N-ext/CP with the ability to bind to the viral silencing suppressor.
DOI: 10.1016/j.virusres.2013.07.013
PubMed: 23911633
Affiliations:
Links toward previous steps (curation, corpus...)
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.</title><author><name sortKey="Sun, Liying" sort="Sun, Liying" uniqKey="Sun L" first="Liying" last="Sun">Liying Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MoA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MoA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Andika, Ida Bagus" sort="Andika, Ida Bagus" uniqKey="Andika I" first="Ida Bagus" last="Andika">Ida Bagus Andika</name></author><author><name sortKey="Shen, Jiangfeng" sort="Shen, Jiangfeng" uniqKey="Shen J" first="Jiangfeng" last="Shen">Jiangfeng Shen</name></author><author><name sortKey="Yang, Di" sort="Yang, Di" uniqKey="Yang D" first="Di" last="Yang">Di Yang</name></author><author><name sortKey="Ratti, Claudio" sort="Ratti, Claudio" uniqKey="Ratti C" first="Claudio" last="Ratti">Claudio Ratti</name></author><author><name sortKey="Chen, Jianping" sort="Chen, Jianping" uniqKey="Chen J" first="Jianping" last="Chen">Jianping Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2013">2013</date><idno type="RBID">pubmed:23911633</idno><idno type="pmid">23911633</idno><idno type="doi">10.1016/j.virusres.2013.07.013</idno><idno type="wicri:Area/Main/Corpus">000446</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Corpus" wicri:corpus="PubMed">000446</idno><idno type="wicri:Area/Main/Curation">000446</idno><idno type="wicri:explorRef" wicri:stream="Main" wicri:step="Curation">000446</idno><idno type="wicri:Area/Main/Exploration">000446</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.</title><author><name sortKey="Sun, Liying" sort="Sun, Liying" uniqKey="Sun L" first="Liying" last="Sun">Liying Sun</name><affiliation wicri:level="1"><nlm:affiliation>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MoA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MoA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Andika, Ida Bagus" sort="Andika, Ida Bagus" uniqKey="Andika I" first="Ida Bagus" last="Andika">Ida Bagus Andika</name></author><author><name sortKey="Shen, Jiangfeng" sort="Shen, Jiangfeng" uniqKey="Shen J" first="Jiangfeng" last="Shen">Jiangfeng Shen</name></author><author><name sortKey="Yang, Di" sort="Yang, Di" uniqKey="Yang D" first="Di" last="Yang">Di Yang</name></author><author><name sortKey="Ratti, Claudio" sort="Ratti, Claudio" uniqKey="Ratti C" first="Claudio" last="Ratti">Claudio Ratti</name></author><author><name sortKey="Chen, Jianping" sort="Chen, Jianping" uniqKey="Chen J" first="Jianping" last="Chen">Jianping Chen</name></author></analytic><series><title level="j">Virus research</title><idno type="eISSN">1872-7492</idno><imprint><date when="2013" type="published">2013</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence (MeSH)</term><term>Base Sequence (MeSH)</term><term>Capsid Proteins (genetics)</term><term>Capsid Proteins (metabolism)</term><term>Molecular Sequence Data (MeSH)</term><term>Peptide Chain Initiation, Translational (MeSH)</term><term>Plant Diseases (virology)</term><term>Protein Binding (MeSH)</term><term>RNA Viruses (genetics)</term><term>RNA Viruses (isolation & purification)</term><term>RNA Viruses (metabolism)</term><term>Sequence Alignment (MeSH)</term><term>Suppression, Genetic (MeSH)</term><term>Triticum (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Alignement de séquences (MeSH)</term><term>Données de séquences moléculaires (MeSH)</term><term>Initiation de la traduction (MeSH)</term><term>Liaison aux protéines (MeSH)</term><term>Maladies des plantes (virologie)</term><term>Protéines de capside (génétique)</term><term>Protéines de capside (métabolisme)</term><term>Suppression génétique (MeSH)</term><term>Séquence d'acides aminés (MeSH)</term><term>Séquence nucléotidique (MeSH)</term><term>Triticum (virologie)</term><term>Virus à ARN (génétique)</term><term>Virus à ARN (isolement et purification)</term><term>Virus à ARN (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de capside</term><term>Virus à ARN</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus à ARN</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>RNA Viruses</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de capside</term><term>Virus à ARN</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Maladies des plantes</term><term>Triticum</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Plant Diseases</term><term>Triticum</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Base Sequence</term><term>Molecular Sequence Data</term><term>Peptide Chain Initiation, Translational</term><term>Protein Binding</term><term>Sequence Alignment</term><term>Suppression, Genetic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Données de séquences moléculaires</term><term>Initiation de la traduction</term><term>Liaison aux protéines</term><term>Suppression génétique</term><term>Séquence d'acides aminés</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Some viruses use alternative translation initiation at non-AUG codons as a strategy to produce multiple proteins during gene expression. Here we show that, using this strategy, Chinese wheat mosaic virus (CWMV; Furovirus) expresses a larger form of coat protein (N-ext/CP) in infected plants. Site-directed mutagenesis and transient expression analysis confirmed that CWMV N-ext/CP is initiated at an upstream in-frame CUG codon at nucleotide position 207-209 of RNA 2, which adds a 39 amino acid (aa) N-terminal extension to the major CP. Interestingly, in planta and in vitro analyses indicated that CWMV N-ext/CP but not CP interacts with the CWMV cysteine-rich protein (CRP), an RNA silencing suppressor. We further determined that the N-terminal 39 aa extension, particularly the 10 aa region immediately upstream of the major CP coding region is responsible for the interaction of N-ext/CP with CRP. In an Agrobacterium co-infiltration assay, co-expression with N-ext/CP did not affect CRP silencing suppression activity. Thus the alternative translation initiation at a CUG codon provides the CWMV N-ext/CP with the ability to bind to the viral silencing suppressor.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">23911633</PMID><DateCompleted><Year>2014</Year><Month>03</Month><Day>19</Day></DateCompleted><DateRevised><Year>2013</Year><Month>09</Month><Day>09</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Electronic">1872-7492</ISSN><JournalIssue CitedMedium="Internet"><Volume>177</Volume><Issue>1</Issue><PubDate><Year>2013</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Virus research</Title><ISOAbbreviation>Virus Res</ISOAbbreviation></Journal><ArticleTitle>The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.</ArticleTitle><Pagination><MedlinePgn>66-74</MedlinePgn></Pagination><ELocationID EIdType="doi" ValidYN="Y">10.1016/j.virusres.2013.07.013</ELocationID><ELocationID EIdType="pii" ValidYN="Y">S0168-1702(13)00235-9</ELocationID><Abstract><AbstractText>Some viruses use alternative translation initiation at non-AUG codons as a strategy to produce multiple proteins during gene expression. Here we show that, using this strategy, Chinese wheat mosaic virus (CWMV; Furovirus) expresses a larger form of coat protein (N-ext/CP) in infected plants. Site-directed mutagenesis and transient expression analysis confirmed that CWMV N-ext/CP is initiated at an upstream in-frame CUG codon at nucleotide position 207-209 of RNA 2, which adds a 39 amino acid (aa) N-terminal extension to the major CP. Interestingly, in planta and in vitro analyses indicated that CWMV N-ext/CP but not CP interacts with the CWMV cysteine-rich protein (CRP), an RNA silencing suppressor. We further determined that the N-terminal 39 aa extension, particularly the 10 aa region immediately upstream of the major CP coding region is responsible for the interaction of N-ext/CP with CRP. In an Agrobacterium co-infiltration assay, co-expression with N-ext/CP did not affect CRP silencing suppression activity. Thus the alternative translation initiation at a CUG codon provides the CWMV N-ext/CP with the ability to bind to the viral silencing suppressor.</AbstractText><CopyrightInformation>Copyright © 2013 Elsevier B.V. All rights reserved.</CopyrightInformation></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Sun</LastName><ForeName>Liying</ForeName><Initials>L</Initials><AffiliationInfo><Affiliation>State Key Laboratory Breeding Base for Zhejiang Sustainable Pest and Disease Control, MoA Key Laboratory for Plant Protection and Biotechnology, Zhejiang Provincial Key Laboratory of Plant Virology, Institute of Virology and Biotechnology, Zhejiang Academy of Agricultural Sciences, Hangzhou 310021, PR China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Andika</LastName><ForeName>Ida Bagus</ForeName><Initials>IB</Initials></Author><Author ValidYN="Y"><LastName>Shen</LastName><ForeName>Jiangfeng</ForeName><Initials>J</Initials></Author><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Di</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Ratti</LastName><ForeName>Claudio</ForeName><Initials>C</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Jianping</ForeName><Initials>J</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2013</Year><Month>07</Month><Day>30</Day></ArticleDate></Article><MedlineJournalInfo><Country>Netherlands</Country><MedlineTA>Virus Res</MedlineTA><NlmUniqueID>8410979</NlmUniqueID><ISSNLinking>0168-1702</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D036022">Capsid Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D036022" MajorTopicYN="N">Capsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010442" MajorTopicYN="Y">Peptide Chain Initiation, Translational</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010935" MajorTopicYN="N">Plant Diseases</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011485" MajorTopicYN="N">Protein Binding</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D012328" MajorTopicYN="N">RNA Viruses</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="Y">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016415" MajorTopicYN="N">Sequence Alignment</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013489" MajorTopicYN="Y">Suppression, Genetic</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014908" MajorTopicYN="N">Triticum</DescriptorName><QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName></MeshHeading></MeshHeadingList><KeywordList Owner="NOTNLM"><Keyword MajorTopicYN="N">Alternative translation initiation</Keyword><Keyword MajorTopicYN="N">CUG codon</Keyword><Keyword MajorTopicYN="N">Chinese wheat mosaic virus</Keyword><Keyword MajorTopicYN="N">Cysteine-rich suppressor</Keyword><Keyword MajorTopicYN="N">Furovirus</Keyword><Keyword MajorTopicYN="N">Larger form coat protein</Keyword></KeywordList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="received"><Year>2013</Year><Month>06</Month><Day>17</Day></PubMedPubDate><PubMedPubDate PubStatus="revised"><Year>2013</Year><Month>07</Month><Day>16</Day></PubMedPubDate><PubMedPubDate PubStatus="accepted"><Year>2013</Year><Month>07</Month><Day>18</Day></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2013</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="pubmed"><Year>2013</Year><Month>8</Month><Day>6</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2014</Year><Month>3</Month><Day>22</Day><Hour>6</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">23911633</ArticleId><ArticleId IdType="pii">S0168-1702(13)00235-9</ArticleId><ArticleId IdType="doi">10.1016/j.virusres.2013.07.013</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><noCountry><name sortKey="Andika, Ida Bagus" sort="Andika, Ida Bagus" uniqKey="Andika I" first="Ida Bagus" last="Andika">Ida Bagus Andika</name><name sortKey="Chen, Jianping" sort="Chen, Jianping" uniqKey="Chen J" first="Jianping" last="Chen">Jianping Chen</name><name sortKey="Ratti, Claudio" sort="Ratti, Claudio" uniqKey="Ratti C" first="Claudio" last="Ratti">Claudio Ratti</name><name sortKey="Shen, Jiangfeng" sort="Shen, Jiangfeng" uniqKey="Shen J" first="Jiangfeng" last="Shen">Jiangfeng Shen</name><name sortKey="Yang, Di" sort="Yang, Di" uniqKey="Yang D" first="Di" last="Yang">Di Yang</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Sun, Liying" sort="Sun, Liying" uniqKey="Sun L" first="Liying" last="Sun">Liying Sun</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Bois/explor/AgrobacTransV1/Data/Main/Exploration
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000444 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd -nk 000444 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Bois
|area= AgrobacTransV1
|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:23911633
|texte= The CUG-initiated larger form coat protein of Chinese wheat mosaic virus binds to the cysteine-rich RNA silencing suppressor.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:23911633" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a AgrobacTransV1
| This area was generated with Dilib version V0.6.38. |  |